Preview - Central Dogma

What Enzymes are Needed?


EXPLORE THE BIOLOGY:

DNA (DeoxyriboNucleic Acid) is made of two strands which wind around each other in a double helix.  When DNA is replicated, the DNA strands are separated and each serves as a template (i.e. the basis for a pattern) for a new double helix.

 

DNA is a polymer.  Polymers (poly = many) are large molecules made of smaller, linked units called monomers (mono = one). 

DNA is made up of many, individual nucleotides (monomers) that are connected to each other to form both single strands and helical, double strands (polymers).  Nucleotides are attached to a growing strand by an enzyme.    

 

Please read the instructions below, before you use the model.

Start by specifying the following settings in the simulator:

DNA-strand-length:  30                             Speed:  Normal

Enzyme labels: checked                           Substitutions:  unchecked

Nucleo labels: unchecked                         Free-nucleosides:  50                   Time-limit:  None
 

When you have chosen the correct settings, press SETUP.

The simulation setup shows the tools you will need to replicate DNA.  You have a strand of Condensed DNAFree Nucleotides and Replication Enzymes:

 

EXPLORE THE BIOLOGY:

Enzymes are proteins that perform chemical reactions in cells.  This simulation shows you four different enzymes that are integral to DNA replication. 

Press GO/STOP to start the simulation! 

You can drag and drop molecules inside the cell using your mouse cursor.


Questions

Please answer the questions below.

Which enzyme unwinds condensed DNA?  

Hint: Try moving the different enzymes to the condensed DNA. 

[Note: you may have to “let an enzyme go” with your mouse in order to see the enzyme work.]


Which enzyme unzips the double stranded DNA? (Use the model to answer.)


What enzyme will attach nucleotides to form double stranded DNA?

Hint:  You will need to move an enzyme and nucleotides into one place together to form new DNA.


Notes

These notes will appear on every page in this lesson so feel free to put anything here you'd like to keep track of.